Membrane conductance and current-voltage relation in the squid axon under voltage-clamp.
نویسندگان
چکیده
,4BSTRACT TASAKI, I. AND C. S. SPYROPOULOS. Membrane conductance and currentvoltage relation in the squid axon under ‘voltage-clamp.’ &L-The conductance of the squid axon membrane under ‘voltage-clamp’ was measured by superposing a sinusoidal wave upon rectangular clamping voltage pulses. It was possible to determine the time course of the emf of the membrane under ‘voltage-clamp’ on a single photographic record showing the membrane current together with the simultaneously recorded membrane conductance. The properties of the membrane in the mixed state, in which only a portion of the axon membrane is in the excited state, were investigated by the same method. The property of the weak variable inward membrane current which preceded the appearance of discrete inward surges was investigated.
منابع مشابه
Current-Voltage Relations in the Lobster Giant Axon Membrane Under Voltage Clamp Conditions
The sucrose-gap method introduced by Stämpfli provides a means for the application of a voltage clamp to the lobster giant axon, which responds to a variety of different experimental procedures in ways quite similar to those reported for the squid axon and frog node. This is particularly true for the behavior of the peak initial current. However, the steady state current shows some differences....
متن کاملDemonstration of Two Stable Potential States in the Squid Giant Axon under Tetraethylammonium Chloride
1. Intracellular injection of tetraethylammonium chloride (TEA) into a giant axon of the squid prolongs the duration of the action potential without changing the resting potential (Fig. 3). The prolongation is sometimes 100-fold or more. 2. The action potential of a giant axon treated with TEA has an initial peak followed by a plateau (Fig. 3). The membrane resistance during the plateau is prac...
متن کاملIonic conductances of squid giant fiber lobe neurons
The cell bodies of the neurons in the giant fiber lobe (GFL) of the squid stellate ganglion give rise to axons that fuse and thereby form the third-order giant axon, whose initial portion functions as the postsynaptic element of the squid giant synapse. We have developed a preparation of dissociated, cultured cells from this lobe and have studied the voltage-dependent conductances using patch-c...
متن کاملNonuniform response in the squid axon membrana under voltage-clamp.
TASAKT, I. AND C. S. SPYROPOULOS. Nonunijorm response in the squid axon membrane under ‘voltage-clamp.’ Am. J. Physiol. 193(2): 309-317. 1958.-By cooling a portion of the squid giant axon at a time, it was shown that the repetitive membrane currents observed under the so-called voltageclamp conditions derived from the main portion of the ‘clamped’ axon membrane and not from the lateral unclampe...
متن کاملDiscrete threshold and repetitive responses in the squid axon under voltage-clamp.
TASAKI, I. AND A. F. BAK. Discrete threshold and repetitive responses in the squid axon under ‘voltage-claw@ By using the so-called voltage-clamp technique, records were obtained indicating the presence of undulatory or oscillatory membrane current under fixed potential difference between the internal and external electrodes. This oscillatory membrane current was observed only when rectangular ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- The American journal of physiology
دوره 193 2 شماره
صفحات -
تاریخ انتشار 1958